我的基础研究兴趣是病毒与宿主细胞的相互作用, specifically related to the replication 和发病机制 of the lentivirus subfamily of retroviruses. Members of this subfamily include the human 和 simian immunodeficiency viruses (HIV 和 SIV, 分别). I have a broad background in molecular virology 和 extensive experience studying retroviruses including an established record of productive research projects in retroviral studies, 包括分子病毒学, 蛋白质组学研究, 和
药物测试. 我在分子病毒学方面也有经验, 包括逆转录病毒和慢病毒基因传递系统以及RNAi和CRISPR.
.
研究的焦点
HIV分子病毒学与宿主细胞相互作用我的基础研究兴趣是病毒与宿主细胞的相互作用. The majority of my work is related to the replication 和发病机制 of the lentivirus subfamily of retroviruses, 包括人类免疫缺陷病毒(HIV)和猿猴免疫缺陷病毒(SIV). 然而,我在病毒学、分子生物学和组学研究方面有广泛的背景. I have worked in the field of virology for over 25 years 和 have an established record of productive research in the areas of molecular virology, 宿主的相互作用, 病毒进化, 蛋白质组学研究, 以及体外药物测试.
HIV是获得性免疫缺陷综合征(AIDS)的病原体。. HIV感染是无法治愈的, but treatment with a combination of anti-retroviral drugs can reduce virus replication to undetectable levels 和 forestall disease progression. 然而,对现有抗逆转录病毒疗法的耐药性仍然令人担忧. Continued success in the repression of HIV replication in infected individuals will require the development of new inhibitors that are effective against drug-resistant strains of virus. Drugs that target novel areas of virus replication have the greatest probability to be effective against such viruses.
My laboratory seeks to comprehend poorly understood aspects of HIV replication by discovery of novel 宿主的相互作用 using systems biology approaches. 最终目标是确定抗病毒治疗的新靶点. HIV复制的所有区域都有潜在的细胞靶标, 包括病毒脱衣, 预集成复合体(PIC)的装配和运输, 病毒转录调控, 病毒的组装和释放.
主要研究领域- 组学研究鉴定新的病毒-宿主相互作用.
- 促进HIV复制的新型宿主细胞因子的表征, 延迟, 和发病机制.
- HIV复制、潜伏期和发病机制的分子生物学
- 在体外 药物测试.
My laboratory is affiliated with the Nebraska Center for 病毒学 a Center of Biomedical Research Excellence formed in the fall of 2000 under the National 研究院 of Health Institutional Development Award program. 该中心与皇冠体育博彩大学的病毒学项目有联系, 内布拉斯加大学林肯分校, 和 the University of Nebraska 地中海ical Center 和 conducts innovative research that addresses fundamental questions about the pathogenesis 和 replication of diverse viral agents that effect human, 动物, 植物健康.